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Abstract No.: ThP-057
Session: Biomarkers
Presentation date: Thu, Aug 31, 2006
Presentation time: 09:50 – 11:20

Cancer Fragmentomics: Identification of Carrier-protein Bound Ovarian Cancer Biomarkers

Mary Lopez1, Scott Kuzdzal1, Alvydas Mikulskis1, David Sarracino2, Martin O'Gorman6, David Fishman7, Kevin Rosenblatt5, Gordon Whiteley4, Emanuel Petricion3, Lance Liotta3

1 PerkinElmer Life, Boston, United States
2 Harvard-Partners, Boston, United States
3 George Mason University, Fairfax, United States
4 NCI, Rockville, United States
5 University of Texas, Southwestern, Dallas, United States
6 Nonlinear Dynamics, Newcastle, United Kingdom
7 NYU, New York, United States

Correspondence address: Scott Kuzdzal, PerkinElmer Life, Molecular Medicine, 549 Albany Street, Boston, MA, 02118 United States.

Keywords: Biomarkers; MALDI; Mass Spectrometry, Ion Cyclotron Resonance; MS/MS.

Novel aspect: Early-stage ovarian cancer biomarkers were identified using a novel membrane based, scaleable carrier-protein bound biomarker enrichment platform that bridges the gap between high-throughput screening and accurate peptide sequencing.

 

Ovarian cancer is diagnosed using ultrasound, computed tomography (CT) scans and magnetic resonance imaging (MRI). A CA-125 test is also used, in conjunction with imaging techniques, for surveillance of women at high-risk for ovarian cancer. These screening modalities are insufficient for detection of early stage ovarian cancer; almost 70 percent of women with common epithelial ovarian cancer are not diagnosed until the disease is in a advanced stage.

We herein report the identification of a protein fragment biomarker panel capable of diagnosing _early stage_ ovarian cancer with very high sensitivity (>93%) and specificity (>97%). We demonstrate the effective combination of high performance, high-throughput MALDI Orthogonal-TOF Mass Spectrometric screening of carrier-protein bound protein fragments and definitive biomarker candidate sequencing via MS/MS. Identifications of several differentially-expressed peptide biomarkers are presented.

 

Copyright © International Mass Spectrometry Foundation (2006)